Human IL-7 recombinant Protein

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Synonyms

Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.

Description of Human IL-7

Recombinant human IL-7 is a 17.4 kDa protein containing 152 amino acid residues. IL-7 is a hematopoietic growth factor, which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive.

Biological Activity

The ED₅₀ of ≤ 0.5 ng/ml was determined by its ability to stimulate the proliferation of mouse 2E8 cells. It corresponds to a specific activity of ≥ 2.0 x 10⁶ units/mg.

Reconstitution

We recommend a quick spin followed by reconstitution in sterile-filtered water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz) and store in working aliquots at -20°C to -80°C.

Stability

The lyophilized protein is stable at room temperature for up to 1 month, at 4°C for up to 6 months, and below -20°C until expiry date. Reconstituted protein is stable for at least 12 months when stored in working aliquots with a carrier protein at -20°C to -80°C. Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EH

Shipped at room temperature

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