Ovine IFN-tau recombinant Protein

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Synonyms

IFN-tau1, Trophoblast protein 1, TP-1, Trophoblastin, Antiluteolysin, Trophoblast antiluteolytic protein, IFN-tau, IFN tau-1.

Description of Ovine IFN-tau

Recombinant Ovine Interferon tau is a 19.91 kDa single, non-glycosylated polypeptide chain containing 172 amino acids. It is also known as TP-1 (trophoblast protein-1) is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. IFN-tau has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-alpha and IFN-beta. The current investigation concerns the effect of recombinant ovine IFN-tau (rOIFN-tau) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN-tau induced tyrosine phosphorylation of Stat1 and upregulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduces the relapse rate in MS is via interference with IFN-?-induced MHC class II expression. IFN-tau was shown to downregulate IFN-?-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in downregulating inflammation in the central nervous system (CNS). IFN-tau did not upregulate the expression of MHC class II on CVE. IFN- tau also inhibited the replication of Theilers virus in CVE.

Biological Activity

The ED₅₀ as determined in a viral resistance assay using bovine kidney MDBK cells was found to be ≥ 1.0 x 10⁷ units/mg.

Reconstitution

We recommend a quick spin followed by reconstitution in sterile water of at least 100µg/ml. This solution can then be further diluted to other aqueous solutions.

Stability

Lyophilized Recombinant Ovine Interferon-tau although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Tyr-Leu-Ser-Arg.

Shipped at room temperature

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